In this paper, a new approach for automatic audio classification using non-negative matrix factorization (NMF) is presented. Training is performed onto each audio class individually, whilst during the test phase each test recording is projected onto the several training matrices. Experiments demonstrating the efficiency of the proposed approach were performed for musical instrument classification. Several perceptual features as well as MPEG-7 descriptors were measured for 300 sound recordings consisting of 6 different musical instrument classes. Subsets of the feature set were selected using branch-and-bound search, in order to obtain the most discriminating features for classification. Several NMF techniques were utilized, namely the standard NMF method, the local NMF, and the sparse NMF. The experiments demonstrate an almost perfect classification