In this paper, we investigate partial words, or finite sequences that may have some undefined positions called holes, of maximum subword complexity. The subword complexity function of a partial word w over a given alphabet of size k assigns to each positive integer n, the number pw(n) of distinct full words over the alphabet that are compatible with factors of length n of w. For positive integers n, h and k, we introduce the concept of a de Bruijn partial word of order n with h holes over an alphabet A of size k, as being a partial word w with h holes over A of minimal length with the property that pw(n) = kn . We are concerned with the following three questions: (1) What is the length of k-ary de Bruijn partial words of order n with h holes? (2) What is an efficient method for generating such partial words? (3) How many