CGHPRO - A comprehensive data analysis tool for array CGH

Background: Array CGH (Comparative Genomic Hybridisation) is a molecular cytogenetic technique for the genome wide detection of chromosomal imbalances. It is based on the cohybridisation of differentially labelled test and reference DNA onto arrays of genomic BAC clones, cDNAs or oligonucleotides, and after correction for various intervening variables, loss or gain in the test DNA can be indicated from spots showing aberrant signal intensity ratios. Now that this technique is no longer confined to highly specialized laboratories and is entering the realm of clinical application, there is a need for a user-friendly software package that facilitates estimates of DNA dosage from raw signal intensities obtained by array CGH experiments, and which does not depend on a sophisticated computational environment. Results: We have developed a user-friendly and versatile tool for the normalization, visualization, breakpoint detection and comparative analysis of array-CGH data. CGHPRO is a stand-a...

• Type: Journal
• Year: 2005
• Where: BMCBI
• Authors: Wei Chen, Fikret Erdogan, H.-Hilger Ropers, Steffen Lenzner, Reinhard Ullmann
• Keywords: Array Cgh, BMCBI 2005, Comparative Genomic Hybridisation, Intensity Ratios