Dinucleotide Step Parameterization of Pre-miRNAs Using Multi-objective Evolutionary Algorithms

MicroRNAs (miRNAs) form a large functional family of small noncoding RNAs and play an important role as posttranscriptional regulators, by repressing the translation of mRNAs. Recently, the processing mechanism of miRNAs has been reported to involve Drosha/DGCR8 complex and Dicer, however, the exact mechanism and molecular principle are still unknown. We thus have tried to understand the related phenomena in terms of the tertiary structure of pre-miRNA. Unfortunately, the tertiary structure of RNA double helix has not been studied sufficiently compared to that of DNA double helix. The tertiary structure of pre-miRNA double helix is determined by 15 types of dinucleotide step (d-step) parameters for three classes of angles, i.e., twist, roll, and tilt. In this study, we estimate the 45 d-step parameters (15 types by 3 classes) using an evolutionary algorithm, under several assumptions inferred from the literature. Considering the trade-off among the four objective functions in our study...

• Type: Conference
• Year: 2007
• Where: EVOW
• Authors: Jin-Wu Nam, In-Hee Lee, Kyu Baek Hwang, Seong-Bae Park, Byoung-Tak Zhang
• Keywords: Artificial Intelligence, Double Helix, EVOW 2007, Processing Mechanism, Tertiary Structure