—This paper reports the results of an experimental and finite element analysis of tibial screw fixation in hamstring ACL reconstruction. The mechanical properties of the bone and tendon graft are obtained from experiments using porcine/calf bones and tendon. The results of the numerical study are compared with those from mechanical testing. Analysis shows that the model may be used to establish the optimum placement of the tunnel in anterior cruciate ligament reconstruction by predicting mechanical parameters such as stress, strain and displacement at regions in the tunnel wall.