As popularity of the Internet continues to grow, it changes the way of computer crime. Number of computer crime increases dramatically in recent years and investigators have been facing the difficulty of admissibility of digital evidence. To solve this problem, we must collect evidence by digital forensics techniques and analyze the digital data, or recover the damaged data. In this research, we integrate several open source digital forensics tools and create a graphic user interface to develop a user-friendly environment for investigators. To avoid evidence loss due to shutdown of target hosts, we use the live analysis technique to collect volatile data with executing commands from an external USB. We also create a live USB so that target hosts can boot from the USB which contains a functional operating system with tools for forensic discovery. Keywords- Digital Forensics, Computer Forensics, Digital Evidence, Live Analysis, Live USB