FiatFlux - a software for metabolic flux analysis from 13C-glucose experiments

Background: Quantitative knowledge of intracellular fluxes is important for a comprehensive characterization of metabolic networks and their functional operation. In contrast to direct assessment of metabolite concentrations, in vivo metabolite fluxes must be inferred indirectly from measurable quantities in 13C experiments. The required experience, the complicated network models, large and heterogeneous data sets, and the time-consuming set-up of highly controlled experimental conditions largely restricted metabolic flux analysis to few expert groups. A conceptual simplification of flux analysis is the analytical determination of metabolic flux ratios exclusively from MS data, which can then be used in a second step to estimate absolute in vivo fluxes. Results: Here we describe the user-friendly software package FiatFlux that supports flux analysis for non-expert users. In the first module, ratios of converging fluxes are automatically calculated from GC-MS-detected 13C-pattern in pr...

• Type: Journal
• Year: 2005
• Where: BMCBI
• Authors: Nicola Zamboni, Eliane Fischer, Uwe Sauer
• Keywords: BMCBI 2005, FLUX, Flux Analysis, Flux Ratios