Increasing Consensus Accuracy in DNA Fragment Assemblies by Incorporating Fluorescent Trace Representations

We present a new method for determining the consensus sequence in DNA fragment assemblies. The new method, Trace-Evidence, directly incorporates aligned ABI trace information into consensus calculations via our previously described representation, Trace-Data Classifications. The new method extracts and sums evidence indicated by the representation to determine consensus calls. Using the Trace-Evidence method results in automatically produced consensus sequences that are more accurate and less ambiguous than those produced with standard majorityvoting methods. Additionally, these improvements are achieved with less coverage than required by the standard methods – using Trace-Evidence and a coverage of only three, errorrates are as low as those with a coverage of over ten sequences.

• Type: Conference
• Year: 1997
• Where: ISMB
• Authors: Carolyn F. Allex, Schuyler F. Baldwin, Jude W. Shavlik, Frederick R. Blattner
• Keywords: Computational Biology, Consensus Calculations, Consensus Sequences, DNA Fragment Assemblies, ISMB 1997