Message-passing for base-calling in sequencing-by-synthesis systems

Performance of DNA sequencing-by-synthesis systems is fundamentally limited by the stochastic nature of the underlying biochemical process. We develop a novel graphical representation of sequencing-by-synthesis which allows for computationally efficient base-calling via message-passing algorithms. Computational studies indicate that the proposed message-passing technique outperforms existing base-calling methods.

• Type: Journal
• Year: 2011
• Where: ICASSP
• Authors: Sang Hyun Lee, Manohar Shamaiah, Haris Vikalo
• Keywords: Biochemical Process, ICASSP 2011, Message-passing Technique, Signal Processing, Stochastic Nature