Multiresolution identification of germ layer components in teratomas derived from human and nonhuman primate embryonic stem cell

We propose a system for identification of germ layer components in teratomas derived from human and nonhuman primate embryonic stem cells. Tissue regeneration and repair, drug testing and discovery, the cure of genetic and developmental syndromes all may rest on the understanding of the biology and behavior of embryonic stem (ES) cells. Within the field of stem cell biology, an ES cell is not considered an ES cell until it can produce a teratoma tumor (the "gold" standard test); a seemingly disorganized mass of tissue derived from all three embryonic germ layers; ectoderm, mesoderm, and endoderm. Identification and quantification of tissue types within teratomas derived from ES cells may expand our knowledge of abnormal and normal developmental programming and the response of ES cells to genetic manipulation and/or toxic exposures. In addition, because of the tissue complexity, identifying and quantifying the tissue is tedious and time consuming, but in turn the teratoma pro...

• Type: Conference
• Year: 2008
• Where: ISBI
• Authors: Amina Chebira, John A. Ozolek, Carlos A. Castro, William G. Jenkinson, Mukta Gore, Ramamurthy Bhagavatula, Irina Khaimovich, Shauna E. Ormon, Christopher S. Navara, Meena Sukhwani, Kyle E. Orwig, Ahmi Ben-Yehudah, Gerald Schatten, Gustavo K. Rohde, Jelena Kovacevic
• Keywords: Embryonic Stem Cells, ES Cells, Germ Layer Components, ISBI 2008, Medical Imaging