A Novel Approach to Neurite Tracing in Fluorescence Microscopy Images

In this paper we propose a new, interactive technique for the segmentation of elongated structures in images. It is based on the so-called live-wire segmentation paradigm and uses a newly developed steerable filter for computing local ridge strength and orientation. We describe the principles of our segmentation technique and present the results of preliminary experiments demonstrating its potential for the tracing of neurites in fluorescence microscopy images. KEY WORDS Biomedical imaging, image segmentation, neurite tracing, fluorescence microscopy.

• Type: Conference
• Year: 2003
• Where: SIP
• Authors: Erik H. W. Meijering, Mathews Jacob, J.-C. Floyd Sarria, Michael Unser
• Keywords: Segmentation Technique, SIP 2003, SIP 2007, So-called Live-wire Segmentation, fluorescence Microscopy