: Threats to information security for healthcare information system increased tremendously. There are various factors that contribute to information security threats, many researchers focused only on certain factors which interest them (e.g., virus attack). Certain factors which may be important remain unexplored. In addition, lack of tools and technologies directed to the limited number of threats traced in healthcare system. Thus, it introduces bias in threat analysis. This study explored the use of biological computational termed Genetic Algorithm (GAs) combined with Cox regression (CoRGA) in identifying a potential threat for healthcare system. The results show that variable described “revelation accidently classified data by staff” is one of the major information security threats for healthcare system. Results were compared with manual analysis using the same data, and it is shows that GAs not only introduces new threats for healthcare system but it indicated similar threats p...