Insider threat is a great challenge for most organizations in today’s digital world. It has received substantial research attention as a significant source of information security threat that could cause more financial losses and damages than any other threats. However, designing an effective monitoring and detection framework is a very challenging task. In this paper, we examine the use of human bio-signals to detect the malicious activities and show that its applicability for insider threats detection. We employ a combination of the electroencephalography (EEG) and the electrocardiogram (ECG) signals to provide a framework for insider threat monitoring and detection. We empirically tested the framework with ten subjects and used several activities scenarios. We found that our framework able to achieve up to 90% detection accuracy of the malicious activities when using the electroencephalography (EEG) signals alone. We then examined the effectiveness of adding the electrocardiogr...