—Recently, broadband analog network coding (ANC) was introduced to utilize high-data rate transmission over the wireless – frequency selective fading – channel. However, ANC requires the knowledge of channel state information (CSI) for self-information removal and coherent signal detection. In this paper, we propose a two-slot pilot-assisted CE for bi-directional broadband ANC. In the first slot, two users transmit their respective pilots to the relay, where the users’ pilot signals are designed to avoid the interference and consequently, allow the relay to estimate the CSIs from both users. During the second slot the relay broadcast its pilot signal to both users that estimate the corresponding CSIs. It was shown by computer simulation that, even with imperfect CSI, the BER performance of broadband ANC gives a satisfactory performance for a low and moderate mobile terminal speed in a frequency-selective fading channel.