Universal DNA tag systems: a combinatorial design scheme

Custom-designed DNA arrays offer the possibility of simultaneously monitoring thousands of hybridization reactions. These arrays show great potential for many medical and scientific applications such as polymorphism analysis and genotyping. Relatively high costs are associated with the need to specifically design and synthesize problemspecific arrays. Recently, an alternative approach was suggested that utilizes fixed, universal arrays. This approach presents an interesting design problem--the arrays should contain as many probes as possible, while minimizing experimental errors caused by cross-hybridization. We use a simple thermodynamic model to cast this design problem in a formal mathematical framework. Employing new combinatorial ideas, we derive an efficient construction for the design problem, and prove that our construction is near-optimal.

• Type: Conference
• Year: 2000
• Where: RECOMB
• Authors: Amir Ben-Dor, Richard M. Karp, Benno Schwikowski, Zohar Yakhini
• Keywords: Computational Biology, Custom-designed DNA Arrays, Problemspecific Arrays, RECOMB 2000, Universal Arrays