Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing

Background: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. Results: We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions. Conclusions: A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research. Background Most proteomics workflows from complex biological matrices require extensive sample processing at peptide or protein level to increase identification coverage. Recently, due to its separation capabilities, ease of use and relatively low cost, OFFGEL isoelectric focussing (IEF) has become a popular tool to fractionate proteins and peptides...

• Type: Journal
• Year: 2010
• Where: BMCBI
• Authors: David-Olivier D. Azulay, Hendrik Neubert, Mireia Fernández Ocaña
• Keywords: BMCBI 2010, Peptide, Peptide Fractionation, Visualisation Tool