Distributed interNet Application (DNA) covers a wide range of topics. DNA is a methodology that specifies how to distribute Internet application on various Web servers. DNA helps to generate scalable, reliable enterprise applications. It provides load-balancing techniques to distribute load on multiple Web servers. This paper describes DNA methodology for a distributed application, which enables better performance, availability and service to clients. This paper also provides comparison of application’s performance and scalability between DNA and non-DNA application. The comparison clearly indicates web server performance improvement using DNA methodology. CPU usage improvement statistics are also provided in this paper. Choosing optimized technology is one of the major criteria in a distributed system to achieve best result. Current major industries are moving towards distributed Internet application solution for global market strategies.