RIFLE: Rapid Identification of Microorganisms by Fragment Length Evaluation

Biological macromoleculesrepresent a valuable source of informationfor the identification and phylogenetic classification of microorganisms.Oneof the mostcommonlyused macromoleculesfor this task is the 16S rDNA. The WWW-basedRIFLE system presented here supports large-scale identification tasks by comparing 16SrDNArestriction patterns to a database of restriction patterns derived fromsequencedatabases. Computingefficency and robustness against experimental errors are gained by emploinga newdistance measurefor restriction patterns, the fragmentlength distance. Results from the application of the system to the identification of unculturedmicroorganismsassociated with the seagrass halophila stipulacea show the reliability of the method.

• Type: Conference
• Year: 1997
• Where: ISMB
• Authors: Henning Hermjakob, Robert Giegerich, Walter Arnold
• Keywords: Computational Biology, ISMB 1997, Large-scale Identification Tasks, Patterns Derived Fromsequencedatabases, Restriction Patterns