Due to the flexibility and adaptability of human, manual handling work is still very important in industry, especially for assembly and maintenance work. Well-designed work operation can improve work efficiency and quality; enhance safety, and lower cost. Most traditional methods for work system analysis need physical mock-up and are time consuming. Digital mockup (DMU) and digital human modeling (DHM) techniques have been developed to assist ergonomic design and evaluation for a specific worker population (e.g. 95 percentile); however, the operation adaptability and adjustability for a specific individual are not considered enough. In this study, a new framework based on motion tracking technique and digital human simulation technique is proposed for motion-time analysis of manual operations. A motion tracking system is used to track a worker's operation while he/she is conducting a manual handling work. The motion data is transferred to a simulation computer for real time digit...