HaptenDB: a comprehensive database of haptens, carrier proteins and anti-hapten antibodies

The key requirement for successful immunochemical assay is the availability of antibodies with high specificity and desired affinity. Small molecules, when used as haptens, are not immunogenic. However, on conjugating with carrier molecule they elicit antibody response. The production of anti-hapten antibodies of desired specificity largely depends on the hapten design (preserving greatly the chemicalstructureand spatialconformation of targetcompound), selectionof the appropriate carrier protein and the conjugation method. This manuscript describes a curated database HaptenDB, where information is collected from published literature and web resources. The current versionofthedatabasehas2021entriesfor1087haptensand25carrier proteins, where each entry provides comprehensive details about (1) nature of the hapten, (2) 2D and 3D structures of haptens, (3) carrier proteins, (4) coupling method, (5) method of anti-hapten antibody production, (6) assay method (used for characterization) and ...

• Type: Journal
• Year: 2006
• Where: BIOINFORMATICS
• Authors: Mahender Kumar Singh, Shilpy Srivastava, G. P. S. Raghava, Grish C. Varshney
• Keywords: Antibodies, BIOINFORMATICS 2006, Carrier Proteins, Hapten