MPrime: efficient large scale multiple primer and oligonucleotide design for customized gene microarrays

Background: Enhancements in sequencing technology have recently yielded assemblies of large genomes including rat, mouse, human, fruit fly, and zebrafish. The availability of large-scale genomic and genic sequence data coupled with advances in microarray technology have made it possible to study the expression of large numbers of sequence products under several different conditions in days where traditional molecular biology techniques might have taken months, or even years. Therefore, to efficiently study a number of gene products associated with a disease, pathway, or other biological process, it is necessary to be able to design primer pairs or oligonucleotides en masse rather than using a time consuming and laborious gene-by-gene method. Results: We have developed an integrated system, MPrime, in order to efficiently calculate primer pairs or specific oligonucleotides for multiple genic regions based on a keyword, gene name, accession number, or sequence fasta format within the ra...

• Type: Journal
• Year: 2005
• Where: BMCBI
• Authors: Eric C. Rouchka, Abdelnaby Khalyfa, Nigel G. F. Cooper
• Keywords: BMCBI 2005, Fruit Fly, Genomes, Primer Pairs