Quantitative phase and amplitude imaging using Differential-Interference Contrast (DIC) microscopy

We present an extension of the development of an alternating minimization (AM) method1 for the computation of a specimen's complex transmittance function (magnitude and phase) from DIC images. The ability to extract both quantitative phase and amplitude information from two rotationally-diverse DIC images (i.e., acquired by rotating the sample) extends previous efforts in computational DIC microscopy that have focused on quantitative phase imaging only. Simulation results show that the inverse problem at hand is sensitive to noise as well as to the choice of the AM algorithm parameters. The AM framework allows constraints and penalties on the magnitude and phase estimates to be incorporated in a principled manner. Towards this end, Green and De Pierro's "log-cosh" regularization penalty is applied to the magnitude of differences of neighboring values of the complex-valued function of the specimen during the AM iterations. The penalty is shown to be convex in the co...

• Type: Journal
• Year: 2009
• Where: CIMAGING
• Authors: Chrysanthe Preza, Joseph A. O'Sullivan
• Keywords: AM Algorithm, CIMAGING 2009, DIC Images, Hardware, Quantitative Phase