Mechanical models for soft human organs are necessary for a variety of medical applications, such as surgical planning, virtual reality surgery simulators, and for diagnostic purposes. An adequate quantitative description of the mechanical behaviour of human organs requires high quality experimental data to be acquired and analyzed. We present a novel technique for the acquisition of such data from soft tissues and its post processing to determine some parameters of the tissue's mechanical properties. A small tube is applied to the target organ and a weak vacuum is generated inside the tube according to a predefined pressure history. A video camera grabs images of the deformation profile of the aspirated tissue, and a pressure sensor measures the correspondent vacuum level. The images are processed and used to inform the fitting of uniaxial and continuum mechanics models. Whilst the aspiration test device has been designed to fulfill the requirements for in-vivo applications, for ...