Limits of performance of quantitative polymerase chain reaction systems

Abstract--Estimation of the DNA copy number in a given biological sample is an important problem in genomics. Quantitative polymerase chain reaction (qPCR) systems detect the target DNA molecules by amplifying their number through a series of thermal cycles and measuring the amount of created amplicons in each cycle. Ideally, the number of target molecules doubles at the end of each cycle. However, in practice, due to biochemical noise the efficiency of the qPCR reaction--defined as the fraction of the target molecules which are successfully copied during a cycle--is

• Type: Journal
• Year: 2010
• Where: TIT
• Authors: Haris Vikalo, Babak Hassibi, Arjang Hassibi
• Keywords: Education, Quantitative Polymerase Chain Reaction, Target Dna Molecules, Target Molecules, TIT 2010