An autostereoscopic display and analysis system has been developed to aid the development of three-dimensional (3-D) cardiac imaging for use during minimally invasive cardiac surgery. We have previously developed a dynamic autostereoscopic display device by integral videography autostereoscopic imaging method with high-resolution multi-projection technique. This paper integrated the high-quality autostereoscopic display system into imageguided cardiac surgery. The human heart was scanned in a sequence of volumetric images representing the motion of the human heart throughout the cardiac cycle, based on dynamic information of heart from CT scanning. Timesequence 3-D image analysis can be particularly useful in cardiac applications. This approach will allow us to acquire the optimum method to produce 3-D cardiac image for planning and guidance of minimally invasive cardiac surgery.