Strategic Business and IT Alignment (SBITA) is still ranked amongst the top concerns of the enterprise’s management executives. Such alignment is an organization-wide issue that influences the company’s overall performance and its assessment is a fundamental input for the enterprise’s managers to make informed decisions on SBITA enhancement possibilities. This paper reports the application of an Enterprise Architecture-based SBITA assessment metamodel in a case study conducted in an intensive IT service enterprise. The case study addresses two research questions: How can be applied the proposed Enterprise Architecture-based SBITA assessment metamodel in enterprises? and What is the quality and use of the results of such application? The authors have published the Enterprise Architecture-based SBITA assessment metamodel as a tool that combines the comprehensive and systematic modeling practices in the field of Enterprise Architecture with the guidance of tested and benchmarked SB...