Abstract. We introduce a new glass tile generation method for simulating Stained Glass using region segmentation algorithm and cubic spline interpolation method. We apply a Mean shift segmentation algorithm to a source image to extract a shape of glass tile. We merge regions by user input and use morphological operation to remove the invalid shape. To make the shape of glass tile, we apply cubic spline interpolation and obtain the leading and the region with smooth boundary. Next, we re-segment the region using the spline curves. Finally we apply the transformed colors to each region to create a whole glass tile.