Amplification of the Gene Ontology annotation of Affymetrix probe sets

Background: The annotations of Affymetrix DNA microarray probe sets with Gene Ontology terms are carefully selected for correctness. This results in very accurate but incomplete annotations which is not always desirable for microarray experiment evaluation. Results: Here we present a protocol to amplify the set of Gene Ontology annotations associated to Affymetrix DNA microarray probe sets using information from related databases. Conclusion: Predicted novel annotations and the evidence producing them can be accessed at Probe2GO: http://www.ogic.ca/p2g. Scripts are available on demand. Background The biological interpretation of DNA microarray experiments to measure gene expression requires contrasting the detected patterns of gene expression (obtained, for example, using a clustering algorithm) with the functions of the genes that correspond to those patterns. Because of the high number of genes interrogated by this technique, human interpretation is difficult and a number of computa...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: Enrique M. Muro, Carolina Perez-Iratxeta, Miguel A. Andrade-Navarro
• Keywords: Affymetrix Dna Microarrays, Annotations, BMCBI 2006, Gene