Analysis of DNA strand-specific differential expression with high density tiling microarrays

Background: DNA microarray technology allows the analysis of genome structure and dynamics at genome-wide scale. Expression microarrays (EMA) contain probes for annotated open reading frames (ORF) and are widely used for the analysis of differential gene expression. By contrast, tiling microarrays (TMA) have a much higher probe density and provide unbiased genome-wide coverage. The purpose of this study was to develop a protocol to exploit the high resolution of TMAs for quantitative measurement of DNA strand-specific differential expression of annotated and non-annotated transcripts. Results: We extensively filtered probes present in Affymetrix Genechip Yeast Genome 2.0 expression and

• Type: Journal
• Year: 2010
• Where: BMCBI
• Authors: Luis Quintales, Mar Sánchez, Francisco Antequera
• Keywords: BMCBI 2010, DNA Microarray Technology, Expression Microarrays, Unbiased Genome-wide Coverage