We describe an empirical investigation into layout criteria that can help with the comprehension of Euler diagrams. Our work is intended to inform automatic Euler diagram layout research by confirming the importance of various Euler diagram aesthetic criteria. The three criteria under investigation were: smoothness, zone area equality and edge closeness. Subjects were asked to interpret diagrams with different combinations of levels for each of the criteria. Results for this investigation indicate that, within the parameters of the study, all three criteria are important for understanding Euler diagrams and we have a preliminary indication of the ordering of importance for the criteria.