—Unlike terrestrial wireless communication which uses radio waves, underwater communication relies on acoustic waves. The long latency and limited bandwidth pose great challenges in underwater Media Access Control (MAC) protocol design. As a result, terrestrial MAC protocols perform inefficiently when deployed directly in an underwater environment. In this paper, we examine how an existing asynchronous handshaking based protocol called Multiple Access Collision Avoidance (MACA) can be adapted for use in multi-hop underwater networks. Three areas of improvement are investigated, namely, the state transition rules, the packet forwarding strategy, and the backoff algorithm. Throughput performance is also evaluated through extensive simulation in multi-hop underwater networks. Due to its simplicity and throughput stability, our proposed MAC protocol can be adopted as a reference MAC protocol for underwater networks, with which a more sophisticated underwater MAC may benchmark its perfor...