MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins

Mitochondria are complex organelles that house essential pathways involved in energy metabolism, ion homeostasis, signalling and apoptosis. To understand mitochondrial pathways in health and disease, it is crucial to have an accurate inventory of the organelle’s protein components. In 2008, we made substantial progress toward this goal by performing in-depth mass spectrometry of mitochondria from 14 organs, epitope tagging/microscopy and Bayesian integration to assemble MitoCarta (www.broadinstitute.org/pubs/MitoCarta): an inventory of genes encoding mitochondrial-localized proteins and their expression across 14 mouse tissues. Using the same strategy we have now reconstructed this inventory separately for human and for mouse based on (i) improved gene transcript models, (ii) updated literature curation, including results from proteomic analyses of mitochondrial subcompartments, (iii) improved homology mapping and (iv) updated versions of all seven original data sets. The updated hu...

• Type: Journal
• Year: 2016
• Where: NAR
• Authors: Sarah E. Calvo, Karl R. Clauser, Vamsi K. Mootha
• Keywords: NAR 2016