poolHiTS: A Shifted Transversal Design based pooling strategy for high-throughput drug screening

Background: A key goal of drug discovery is to increase the throughput of small molecule screens without sacrificing screening accuracy. High-throughput screening (HTS) in drug discovery involves testing a large number of compounds in a biological assay to identify active compounds. Normally, molecules from a large compound library are tested individually to identify the activity of each molecule. Usually a small number of compounds are found to be active, however the presence of false positive and negative testing errors suggests that this one-drug one-assay screening strategy can be significantly improved. Pooling designs are testing schemes that test mixtures of compounds in each assay, thereby generating a screen of the whole compound library in fewer tests. By repeatedly testing compounds in different combinations, pooling designs also allow for errorcorrection. These pooled designs, for specific experiment parameters, can be simply and efficiently created using the Shifted Trans...

• Type: Journal
• Year: 2008
• Where: BMCBI
• Authors: Raghunandan M. Kainkaryam, Peter J. Woolf
• Keywords: BMCBI 2008, Compound Library, Drug Discovery, Pooling Strategy