Predicted mouse peroxisome-targeted proteins and their actual subcellular locations

Background: The import of most intraperoxisomal proteins is mediated by peroxisome targeting signals at their C-termini (PTS1) or N-terminal regions (PTS2). Both signals have been integrated in subcellular location prediction programs. However their present performance, particularly of PTS2-targeting did not seem fitting for large-scale screening of sequences. Results: We modified an earlier reported PTS1 screening method to identify PTS2-containing mouse candidates using a combination of computational and manual annotation. For rapid confirmation of five new PTS2- and two previously identified PTS1-containing candidates we developed the new cell line CHO-perRed which stably expresses the peroxisomal marker dsRed

• Type: Journal
• Year: 2008
• Where: BMCBI
• Authors: Yumi Mizuno, Igor Kurochkin, Marlis Herberth, Yasushi Okazaki, Christian Schönbach
• Keywords: BMCBI 2008, Earlier Reported Pts1, Peroxisome Targeting Signals, Subcellular Location Prediction