Serial Algorithm for high-speed autofocusing of cells using Depth From Diffraction (DFDi) method

Abstract— In this paper, a new serial algorithm for highspeed autofocusing of cells is proposed. The proposed algorithm extracts depth information of target cells from their diffraction patterns. High-speed focusing of yeast cells with 20 ms response time was demonstrated. Continuous autofocusing of yeast cells with a scanning microscope was also demonstrated. The successful continuous autofocusing suggests that image-based highthroughput measurement of cells could be realized using the proposed algorithm.

• Type: Conference
• Year: 2008
• Where: ICRA
• Authors: Soshiro Makise, Hiromasa Oku, Masatoshi Ishikawa
• Keywords: Continuous Autofocusing, ICRA 2008, Robotics, Target Cells, Yeast Cells