Systematic identification of yeast cell cycle transcription factors using multiple data sources

Background: Eukaryotic cell cycle is a complex process and is precisely regulated at many levels. Many genes specific to the cell cycle are regulated transcriptionally and are expressed just before they are needed. To understand the cell cycle process, it is important to identify the cell cycle transcription factors (TFs) that regulate the expression of cell cycle-regulated genes. Results: We developed a method to identify cell cycle TFs in yeast by integrating current ChIPchip, mutant, transcription factor binding site (TFBS), and cell cycle gene expression data. We identified 17 cell cycle TFs, 12 of which are known cell cycle TFs, while the remaining five (Ash1, Rlm1, Ste12, Stp1, Tec1) are putative novel cell cycle TFs. For each cell cycle TF, we assigned specific cell cycle phases in which the TF functions and identified the time lag for the TF to exert regulatory effects on its target genes. We also identified 178 novel cell cycle-regulated genes, among which 59 have unknown fun...

• Type: Journal
• Year: 2008
• Where: BMCBI
• Authors: Wei-Sheng Wu, Wen-Hsiung Li
• Keywords: BMCBI 2008, Cell Cycle, Cell Cycle Tfs, Eukaryotic Cell Cycle