Transcriptomic response to differentiation induction

Background: Microarrays used for gene expression studies yield large amounts of data. The processing of such data typically leads to lists of differentially-regulated genes. A common terminal data analysis step is to map pathways of potentially interrelated genes. Methods: We applied a transcriptomics analysis tool to elucidate the underlying pathways of leukocyte maturation at the genomic level in an established cellular model of leukemia by examining time-course data in two subclones of U-937 cells. Leukemias such as Acute Promyelocytic Leukemia (APL) are characterized by a block in the hematopoietic stem cell maturation program at a point when expansion of clones which should be destined to mature into terminallydifferentiated effector cells get locked into endless proliferation with few cells reaching maturation. Treatment with retinoic acid, depending on the precise genomic abnormality, often releases the responsible promyelocytes from this blockade but clinically can yield adver...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: G. W. Patton, Robert M. Stephens, I. A. Sidorov, X. Xiao, Richard A. Lempicki, D. S. Dimitrov, Robert H. Shoemaker, G. Tudor
• Keywords: BMCBI 2006, Precise Genomic Abnormality, Retinoic Acid, Stem Cell Maturation