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BMCBI
2007
174views more  BMCBI 2007»
15 years 2 months ago
Normalization method for metabolomics data using optimal selection of multiple internal standards
Background: Success of metabolomics as the phenotyping platform largely depends on its ability to detect various sources of biological variability. Removal of platform-specific so...
Marko Sysi-Aho, Mikko Katajamaa, Laxman Yetukuri, ...
118
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BMCBI
2005
85views more  BMCBI 2005»
15 years 2 months ago
Processing methods for differential analysis of LC/MS profile data
Background: Liquid chromatography coupled to mass spectrometry (LC/MS) has been widely used in proteomics and metabolomics research. In this context, the technology has been incre...
Mikko Katajamaa, Matej Oresic
BMCBI
2005
137views more  BMCBI 2005»
15 years 2 months ago
A standard curve based method for relative real time PCR data processing
Background: Currently real time PCR is the most precise method by which to measure gene expression. The method generates a large amount of raw numerical data and processing may no...
Alexey Larionov, Andreas Krause, William Miller
98
Voted
BMCBI
2010
106views more  BMCBI 2010»
15 years 2 months ago
Selection of optimal reference genes for normalization in quantitative RT-PCR
Background: Normalization in real-time qRT-PCR is necessary to compensate for experimental variation. A popular normalization strategy employs reference gene(s), which may introdu...
Inna Chervoneva, Yanyan Li, Stephanie Schulz, Sean...
BMCBI
2010
97views more  BMCBI 2010»
14 years 11 months ago
Preprocessing of gene expression data by optimally robust estimators
Background: The preprocessing of gene expression data obtained from several platforms routinely includes the aggregation of multiple raw signal intensities to one expression value...
Matthias Kohl, Hans-Peter Deigner