Nuclear staining and relative distance for quantifying epidermal differentiation in biomarker expression profiling

Background: The epidermal physiology results from a complex regulated homeostasis of keratinocyte proliferation, differentiation and death and is tightly regulated by a specific protein expression during cellular maturation. Cellular in silico models are considered a promising and inevitable tool for the understanding of this complex system. Hence, we need to incorporate the information of the differentiation dependent protein expression in cell based systems biological models of tissue homeostasis. Such methods require measuring tissue differentiation quantitatively while correlating it with biomarker expression intensities. Results: Differentiation of a keratinocyte is characterized by its continuously changing morphology concomitant with its movement from the basal layer to the surface, leading to a decreased average nuclei density throughout the tissue. Based thereon, we designed and evaluated three different mathematical measures (nuclei based, distance based, and joint approach)...

• Type: Journal
• Year: 2008
• Where: BMCBI
• Authors: Thora Pommerencke, Thorsten Steinberg, Hartmut Dickhaus, Pascal Tomakidi, Niels Grabe
• Keywords: BMCBI 2008, Complex Regulated Homeostasis, Joint Approach, Protein Expression