An improved procedure for gene selection from microarray experiments using false discovery rate criterion

Background: A large number of genes usually show differential expressions in a microarray experiment with two types of tissues, and the p-values of a proper statistical test are often used to quantify the significance of these differences. The genes with small p-values are then picked as the genes responsible for the differences in the tissue RNA expressions. One key question is what should be the threshold to consider the p-values small. There is always a trade off between this threshold and the rate of false claims. Recent statistical literature shows that the false discovery rate (FDR) criterion is a powerful and reasonable criterion to pick those genes with differential expression. Moreover, the power of detection can be increased by knowing the number of nondifferential expression genes. While this number is unknown in practice, there are methods to estimate it from data. The purpose of this paper is to present a new method of estimating this number and use it for the FDR procedu...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: James J. Yang, Mark C. K. Yang
• Keywords: BMCBI 2006, Expressed Genes, P-values, Proper Statistical Test