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BMCBI
2006

Accurate and unambiguous tag-to-gene mapping in serial analysis of gene expression

13 years 11 months ago
Accurate and unambiguous tag-to-gene mapping in serial analysis of gene expression
Background: In this study, we present a robust and reliable computational method for tag-togene assignment in serial analysis of gene expression (SAGE). The method relies on current genome information and annotation, incorporation of several new features, and key improvements over alternative methods, all of which are important to determine gene expression levels more accurately. The method provides a complete annotation of potential virtual SAGE tags within a genome, along with an estimation of their confidence for experimental observation that ranks tags that present multiple matches in the genome. Results: We applied this method to the Saccharomyces cerevisiae genome, producing the most thorough and accurate annotation of potential virtual SAGE tags that is available today for this organism. The usefulness of this method is exemplified by the significant reduction of ambiguous cases in existing experimental SAGE data. In addition, we report new insights from the analysis of existin...
Rodrigo Malig, Cristian Varela, Eduardo Agosin, Fr
Added 10 Dec 2010
Updated 10 Dec 2010
Type Journal
Year 2006
Where BMCBI
Authors Rodrigo Malig, Cristian Varela, Eduardo Agosin, Francisco Melo
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