Laboratory Information Management Software for genotyping workflows: applications in high throughput crop genotyping

Background: With the advances in DNA sequencer-based technologies, it has become possible to automate several steps of the genotyping process leading to increased throughput. To efficiently handle the large amounts of genotypic data generated and help with quality control, there is a strong need for a software system that can help with the tracking of samples and capture and management of data at different steps of the process. Such systems, while serving to manage the workflow precisely, also encourage good laboratory practice by standardizing protocols, recording and annotating data from every step of the workflow. Results: A laboratory information management system (LIMS) has been designed and implemented at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) that meets the requirements of a moderately high throughput molecular genotyping facility. The application is designed as modules and is simple to learn and use. The application leads the user throug...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: B. Jayashree, Praveen T. Reddy, Y. Leeladevi, Jonathan H. Crouch, V. Mahalakshmi, Hutokshi K. Buhariwalla, K. E. Eshwar, Emma Mace, Rolf Folksterma, S. Senthilvel, Rajeev K. Varshney, K. Seetha, R. Rajalakshmi, V. P. Prasanth, Subhash Chandra, L. Swarupa, P. SriKalyani, David A. Hoisington
• Keywords: BMCBI 2006, Dna Samples, DNA Sequencer-based Technologies, Genotypic Data