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CANDC
2006
ACM

Why substrate depletion has apparent first-order kinetics in enzymatic digestion

13 years 11 months ago
Why substrate depletion has apparent first-order kinetics in enzymatic digestion
A number of enzyme digestion assays show apparent first-order kinetics of reactant depletion. There are four possible explanations of this phenomenon: (i) the reaction is dominated by a first-order limiting step, (ii) the digestion follows a pseudo-first-order kinetics under the excess of a reactant species, (iii) the first-order kinetics is only applicable to the slow transient of the reaction, or (iv) the aggregate behavior of the reaction pathway produces behavior indistinguishable from the first-order kinetics. In this paper, we investigate the kinetics for protein digestion by formulating rate equations for two proposed mechanisms, namely the one-by-one mechanism and the zipper mechanism. Our analysis shows that the kinetics of protein digestion follows apparent first-order kinetics irrespective of the mechanism for low initial substrate concentration compared to the initial enzyme concentration. Also, our results provide an explanation for experimental observations and suggest a...
Jeyaraman Srividhya, Santiago Schnell
Added 11 Dec 2010
Updated 11 Dec 2010
Type Journal
Year 2006
Where CANDC
Authors Jeyaraman Srividhya, Santiago Schnell
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