Discover protein sequence signatures from protein-protein interaction data

Background: The development of high-throughput technologies such as yeast two-hybrid systems and mass spectrometry technologies has made it possible to generate large protein-protein interaction (PPI) datasets. Mining these datasets for underlying biological knowledge has, however, remained a challenge. Results: A total of 3108 sequence signatures were found, each of which was shared by a set of guest proteins interacting with one of 944 host proteins in Saccharomyces cerevisiae genome. Approximately 94% of these sequence signatures matched entries in InterPro member databases. We identified 84 distinct sequence signatures from the remaining 172 unknown signatures. The signature sharing information was then applied in predicting sub-cellular localization of yeast proteins and the novel signatures were used in identifying possible interacting sites. Conclusion: We reported a method of PPI data mining that facilitated the discovery of novel sequence signatures using a large PPI dataset ...

• Type: Journal
• Year: 2005
• Where: BMCBI
• Authors: Jianwen Fang, Ryan J. Haasl, Yinghua Dong, Gerald H. Lushington
• Keywords: BMCBI 2005, Large Protein-protein Interaction, Protein, Signatures