galaxieEST: addressing EST identity through automated phylogenetic analysis

Background: Research involving expressed sequence tags (ESTs) is intricately coupled to the existence of large, well-annotated sequence repositories. Comparatively complete and satisfactory annotated public sequence libraries are, however, available only for a limited range of organisms, rendering the absence of sequences and gene structure information a tangible problem for those working with taxa lacking an EST or genome sequencing project. Paralogous genes belonging to the same gene family but distinguished by derived characteristics are particularly prone to misidentification and erroneous annotation; high but incomplete levels of sequence similarity are typically difficult to interpret and have formed the basis of many unsubstantiated assumptions of orthology. In these cases, a phylogenetic study of the query sequence together with the most similar sequences in the database may be of great value to the identification process. In order to facilitate this laborious procedure, a pro...

• Type: Journal
• Year: 2004
• Where: BMCBI
• Authors: R. Henrik Nilsson, Balaji Rajashekar, Karl-Henrik Larsson, Björn M. Ursing
• Keywords: BMCBI 2004, Phylogenetic Analysis, Public Sequence Libraries, Well-annotated Sequence Repositories