Calibration and assessment of channel-specific biases in microarray data with extended dynamical range

Background: Non-linearities in observed log-ratios of gene expressions, also known as intensity dependent log-ratios, can often be accounted for by global biases in the two channels being compared. Any step in a microarray process may introduce such offsets and in this article we study the biases introduced by the microarray scanner and the image analysis software. Results: By scanning the same spotted oligonucleotide microarray at different photomultiplier tube (PMT) gains, we have identified a channel-specific bias present in two-channel microarray data. For the scanners analyzed it was in the range of 15

• Type: Journal
• Year: 2004
• Where: BMCBI
• Authors: Henrik Bengtsson, Göran Jönsson, Johan Vallon-Christersson
• Keywords: Bias, Biases, BMCBI 2004, Microarray Scanners