Effect of various normalization methods on Applied Biosystems expression array system data

Background: DNA microarray technology provides a powerful tool for characterizing gene expression on a genome scale. While the technology has been widely used in discovery-based medical and basic biological research, its direct application in clinical practice and regulatory decision-making has been questioned. A few key issues, including the reproducibility, reliability, compatibility and standardization of microarray analysis and results, must be critically addressed before any routine usage of microarrays in clinical laboratory and regulated areas can occur. In this study we investigate some of these issues for the Applied Biosystems Human Genome Survey Microarrays. Results: We analyzed the gene expression profiles of two samples: brain and universal human reference (UHR), a mixture of RNAs from 10 cancer cell lines, using the Applied Biosystems Human Genome Survey Microarrays. Five technical replicates in three different sites were performed on the same total RNA samples according...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: Catalin C. Barbacioru, Yulei Wang, Roger D. Canales, Yongming A. Sun, David N. Keys, Frances Chan, Karen A. Poulter, Raymond R. Samaha
• Keywords: Biosystems Human Genome, BMCBI 2006, Gene Expression, Genome Survey Microarrays