A methodology for global validation of microarray experiments

Background: DNA microarrays are popular tools for measuring gene expression of biological samples. This ever increasing popularity is ensuring that a large number of microarray studies are conducted, many of which with data publicly available for mining by other investigators. Under most circumstances, validation of differential expression of genes is performed on a gene to gene basis. Thus, it is not possible to generalize validation results to the remaining majority of non-validated genes or to evaluate the overall quality of these studies. Results: We present an approach for the global validation of DNA microarray experiments that will allow researchers to evaluate the general quality of their experiment and to extrapolate validation results of a subset of genes to the remaining non-validated genes. We illustrate why the popular strategy of selecting only the most differentially expressed genes for validation generally fails as a global validation strategy and propose random-strati...

• Type: Journal
• Year: 2006
• Where: BMCBI
• Authors: Mathieu Miron, Owen Z. Woody, Alexandre Marcil, Carl Murie, Robert Sladek, Robert Nadon
• Keywords: BMCBI 2006, DNA Microarray, Gene, Gene Expression