A new framework dealing with motion estimation in transparent images is presented. It relies on a block-oriented estimation involving an efficient multiresolution minimization. A downhill simplex method provides an appropriate initialization to this scheme. The estimated velocity vectors are greatly improved by an original postprocessing stage which performs a single motion estimation on differences of warped images. Finally, a regularization step is carried out. It is demonstrated on a large set of simulations that a quarter-pixel accuracy can be attained on noise-free images. The case of noisy images is also addressed and provides satisfactory results, even in the case of low-contrasted medical images. An example on real clinical images is also reported with promising results.