A novel approach to sequence validating protein expression clones with automated decision making

Background: Whereas the molecular assembly of protein expression clones is readily automated and routinely accomplished in high throughput, sequence verification of these clones is still largely performed manually, an arduous and time consuming process. The ultimate goal of validation is to determine if a given plasmid clone matches its reference sequence sufficiently to be "acceptable" for use in protein expression experiments. Given the accelerating increase in availability of tens of thousands of unverified clones, there is a strong demand for rapid, efficient and accurate software that automates clone validation. Results: We have developed an Automated Clone Evaluation (ACE) system – the first comprehensive, multi-platform, web-based plasmid sequence verification software package. ACE automates the clone verification process by defining each clone sequence as a list of multidimensional discrepancy objects, each describing a difference between the clone and its expected...

• Type: Journal
• Year: 2007
• Where: BMCBI
• Authors: Elena Taycher, Andreas Rolfs, Yanhui Hu, Dongmei Zuo, Stephanie E. Mohr, Janice Williamson, Joshua LaBaer
• Keywords: Acceptance Criteria, BMCBI 2007, Clones, Sequence Verification